Application

endometrial function, growth factor production, conception rate, cell adherence, uterine lining, maintenance of uterine patency, uterine contraction

Cell Line Origin

Uterus

Components

Bovine Endometrial Basal Media containing 10% FBS & 10% DMSO

General description

BEnEpC provide an excellent model system to study many aspects of epithelial function and disease, especially those related to the endometrium and pregnancy-related signaling, such as seminal plasma induced prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 release by endometrial cells (Norrby, 2012; Madej, 2012; Malgorzata, 2013), or the role of PLA in mediating oxytocin-stimulated production of prostaglandins (Tithof, 2007). BEnEpC have also been used to demonstrate the association between variants of gap junctions related genes and conception rate (Sugimoto, 2013), and to investigate cellular adherence and cytotoxicity of Histophilus somni (Hoshinoo, 2009).

Preparation Note

2nd passage, >500,000 cells in Bovine Endometrial Basal Media containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the BEnEpC Culture Protocol.